Study on the Effect of N/P Ratio and Cultivation Conditions on Biomass Growth and Phycocyanin Production of Cyanobacterium Oscillatoria sp.


  • Cam Van T. Do HaUI Institute of Technology, Hanoi University of Industry (HaUI), 298 Cau Dien, Bac Tu Liem, Hanoi, Vietnam
  • Thu Phuong T. Nguyen Faculty of Chemical Technology, Hanoi University of Industry (HaUI), 298 Cau Dien, Bac Tu Liem, Hanoi, Vietnam
  • Van Toan Lam Environment Faculty, Hanoi University of Natural Resource and Environment, No 41 A Phu Dien Road, Bac Tu Liem, Hanoi, Vietnam
  • Mai T. Dang Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
  • Hai Yen Nguyen Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
  • Thu Uyen T. Bui Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
  • Thuan Dang Tran Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam



Oscillatoria sp., N/P ratio, Aeration, Light intensity, Biomass, Phycocyanin


Phycocyanin is widely used as a natural colourant in food and cosmetic industries because of its inherent blue colour. It can be incorporated into healthy foods because of its physiological properties, such as antioxidant, anti-inflammatory and liver-protective activities. Cyanobacteria has been regarded as potential phycocyanin producers. Therefore, because of these benefits, many researchers have focused on exploring novel cyanobacteria for mass production of biomass for phycocyanin extraction and purification. In this study, a newly isolated cyanobacterium Oscillatoria sp. was used to investigate its growth and phycocyanin production in BG-11 medium under different N/P ratios (20/1, 25/1, 30/1, 35/1, 40/1, and 45/ 1), light intensities (6600, 13200 and 19700 lux), with aeration and without aeration conditions. The dependent responses evaluated were optical density, phycocyanin content and purity of the cyanobacterium culture. Results revealed that optimal growth of Oscillatoria sp. observed in BG-11 medium was N/P = 35/1, the light intensity of 13200 lux with aeration and achieving an optical density of 5.0 Abs. In addition, the highest phycocyanin content determined for Oscillatoria sp. was 148 mg/g in BG-11 medium having N/P of 35/1 under a light intensity of 19700 lux and aeration. Moreover, the highest phycocyanin purity determined for Oscillatoria sp. was 1.4. The Oscillatoria sp. was ranked as the top phycocyanin producer among cyanobacteria reported in the literature.


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