Bactericidal Quantitative Suspension Assay of Chlorhexidine-Based Disinfectant according to EN Standard 13727

Authors

  • Çinel Köksal Karayildirim Ege University, Science Faculty, Biology Department, 35100 Bornova-Izmir, Turkey
  • Ebru Sanci Ege University Center for Drug Research & Development and Pharmacokinetic Applications, 35100 Bornova-Izmir, Turkey

DOI:

https://doi.org/10.31437/2309-4435.2021.09.1

Keywords:

 EN 13737, Bactericidal activity, Chlorhexidine, Biocides, Escherichia coli K12, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus hirae

Abstract

Purpose: Hand hygiene with biocidal products is an effective strategy for preventing healthcare-associated infection in medical areas. It is a first-line defense in the prevention and control of microbial growth. In comparison to biocide efficacy, methods for biocide susceptibility testing of microorganisms have to be standardized for routine use. One of the aims of this study was to formulate a chlorhexidine-based hand disinfectant as a biocidal product with a low and effective concentration.

Methods: This biocidal product was tested against E. coli K12, P. aeruginosa, S. aureus, and E. hirae to evaluate their susceptibility at different concentrations of chlorhexidine (0.1 %, 0.04 %) according to European (EN) Standard.

Results: The results of the dilution-neutralization method indicated that 0.1 % chlorhexidine-based disinfectant represented strong antibacterial activity with a 5 decimal logarithmic reduction under clean conditions against four reference strains. All experimental analyses and conditions were supported by pre-validation and verification studies


Conclusion: This was the first study on chlorhexidine-based biocidal product analysis which was conducted according to the EN 13727 Standard.

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Published

2021-03-26

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